In DNA and RNA, the nitrogen atom is present

Question:

In DNA and RNA, the nitrogen atom is present in the ring system. Can the Kjeldahl method be used for the estimation of nitrogen present in these? Give reasons.

Solution:

The Kjeldahl method cannot be used for the estimation of nitrogen present in DNA and RNA because nitrogen cannot be converted into ammonium sulphate in the conditions provided in this method. The difficulty in decomposing ring structures limits this test for DNA and RNA.

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