Question:
Find out how Melvin Calvin worked out the complete biosynthetic pathway for synthesis of sugar.
Solution:
The following measures were used: carbon dioxide, a radioactive isotope of carbon, was labelled C14 and was supplied to the plants for this experiment. The path C14
was then monitored and analysed. After the experiment on live plant got over, the plant was killed and the labelled compound was extracted from the dead plant and b
ased on the observation in both live and dead Calvin and his colleagues found the pathway.